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Key stages of Medicago lupulina plant development ( A ), fresh weight of roots ( B ), mycorrhizal growth response for root fresh weight ( C ) per one M. lupulina plant inoculated with R. irregularis , mycorrhizal intensity, M ( D ). Symbol: red tick indicates important developmental stages. “a”, “b”, “c” et al.—different letters indicate significant differences within the same MGR parameter (ANOVA and Tukey’s test; p < 0.05). The average values with standard errors are presented.

Journal: Plants

Article Title: The Effect of Arbuscular Mycorrhizal Fungus and Phosphorus Treatment on Root Metabolome of Medicago lupulina During Key Stages of Development

doi: 10.3390/plants14172685

Figure Lengend Snippet: Key stages of Medicago lupulina plant development ( A ), fresh weight of roots ( B ), mycorrhizal growth response for root fresh weight ( C ) per one M. lupulina plant inoculated with R. irregularis , mycorrhizal intensity, M ( D ). Symbol: red tick indicates important developmental stages. “a”, “b”, “c” et al.—different letters indicate significant differences within the same MGR parameter (ANOVA and Tukey’s test; p < 0.05). The average values with standard errors are presented.

Article Snippet: Medicago lupulina L. subsp. vulgaris Koch MlS-1 line, characterized by high AM symbiotic efficiency (mycorrhizal growth response (MGR)), was used under conditions of deficiency or abundance of Pi.

Techniques:

Ordination of metabolite profiles of M. lupulina roots at different development stages. PCA score plot ( A ). Multidimensional scaling using Spearman’s distance (1 − r ) ( B ). The points correspond to the profiles of metabolites; % (“24.1%”and “33.7%”) is the proportion of variance associated with the principal components (PC); ellipses are 90% confidence intervals.

Journal: Plants

Article Title: The Effect of Arbuscular Mycorrhizal Fungus and Phosphorus Treatment on Root Metabolome of Medicago lupulina During Key Stages of Development

doi: 10.3390/plants14172685

Figure Lengend Snippet: Ordination of metabolite profiles of M. lupulina roots at different development stages. PCA score plot ( A ). Multidimensional scaling using Spearman’s distance (1 − r ) ( B ). The points correspond to the profiles of metabolites; % (“24.1%”and “33.7%”) is the proportion of variance associated with the principal components (PC); ellipses are 90% confidence intervals.

Article Snippet: Medicago lupulina L. subsp. vulgaris Koch MlS-1 line, characterized by high AM symbiotic efficiency (mycorrhizal growth response (MGR)), was used under conditions of deficiency or abundance of Pi.

Techniques:

Differential accumulation of metabolites (content relative to AM-P+) at different vegetative stages of M. lupulina development. Heat map of the difference between the average values (normalized and logarithmic) of the metabolites content in the roots of plants in AM+P+ (inoculated with AMF and with phosphorus treatment), in AM+P- (inoculated with AMF and without phosphorus treatment), in AM-P- (without AMF inoculation and without phosphorus treatment) and the control variant AM-P+ (without AMF inoculation with phosphorus treatment). The differentially accumulated metabolites were selected according to VIP > 1 predictive components of the OPLS_DA models. The red color corresponds to a higher level relative to AM-P+. Abbreviations: compsug—complex sugar, molecules with carbohydrate moieties, hexacid—hexonic acid, hexalc—hexose alcohol, pentacid—pentonic acid, pentalc—pentose alcohol, hexose-P—hexose phosphate, MG—monoacylglycerol, FA—fatty acid, GABA—gamma-aminobutanoic acid, RI—retention index.

Journal: Plants

Article Title: The Effect of Arbuscular Mycorrhizal Fungus and Phosphorus Treatment on Root Metabolome of Medicago lupulina During Key Stages of Development

doi: 10.3390/plants14172685

Figure Lengend Snippet: Differential accumulation of metabolites (content relative to AM-P+) at different vegetative stages of M. lupulina development. Heat map of the difference between the average values (normalized and logarithmic) of the metabolites content in the roots of plants in AM+P+ (inoculated with AMF and with phosphorus treatment), in AM+P- (inoculated with AMF and without phosphorus treatment), in AM-P- (without AMF inoculation and without phosphorus treatment) and the control variant AM-P+ (without AMF inoculation with phosphorus treatment). The differentially accumulated metabolites were selected according to VIP > 1 predictive components of the OPLS_DA models. The red color corresponds to a higher level relative to AM-P+. Abbreviations: compsug—complex sugar, molecules with carbohydrate moieties, hexacid—hexonic acid, hexalc—hexose alcohol, pentacid—pentonic acid, pentalc—pentose alcohol, hexose-P—hexose phosphate, MG—monoacylglycerol, FA—fatty acid, GABA—gamma-aminobutanoic acid, RI—retention index.

Article Snippet: Medicago lupulina L. subsp. vulgaris Koch MlS-1 line, characterized by high AM symbiotic efficiency (mycorrhizal growth response (MGR)), was used under conditions of deficiency or abundance of Pi.

Techniques: Control, Variant Assay

Differential accumulation of metabolites (content relative to AM-P+) at different generative stages of M. lupulina development. Heat map of the difference between the average values (normalized and logarithmic) of the metabolite content in the roots of plants in AM+P+ (inoculated with AMF and with phosphorus treatment), in AM+P- (inoculated with AMF and without phosphorus treatment), in AM-P- (without AMF inoculation and without phosphorus treatment) and the control variant AM-P+ (without AMF inoculation with phosphorus treatment). The differentially accumulated metabolites were selected according to VIP > 1 predictive components of the OPLS_DA models. The red color corresponds to a higher level relative to AM-P+. Abbreviations: compsug—complex sugar, molecules with carbohydrate moieties, hexacid—hexonic acid, hexalc—hexose alcohol, pentacid—pentonic acid, pentalc—pentose alcohol, hexose-P—hexose phosphate, MG—monoacylglycerol, FA—fatty acid, GABA—gamma-aminobutanoic acid, RI—retention index.

Journal: Plants

Article Title: The Effect of Arbuscular Mycorrhizal Fungus and Phosphorus Treatment on Root Metabolome of Medicago lupulina During Key Stages of Development

doi: 10.3390/plants14172685

Figure Lengend Snippet: Differential accumulation of metabolites (content relative to AM-P+) at different generative stages of M. lupulina development. Heat map of the difference between the average values (normalized and logarithmic) of the metabolite content in the roots of plants in AM+P+ (inoculated with AMF and with phosphorus treatment), in AM+P- (inoculated with AMF and without phosphorus treatment), in AM-P- (without AMF inoculation and without phosphorus treatment) and the control variant AM-P+ (without AMF inoculation with phosphorus treatment). The differentially accumulated metabolites were selected according to VIP > 1 predictive components of the OPLS_DA models. The red color corresponds to a higher level relative to AM-P+. Abbreviations: compsug—complex sugar, molecules with carbohydrate moieties, hexacid—hexonic acid, hexalc—hexose alcohol, pentacid—pentonic acid, pentalc—pentose alcohol, hexose-P—hexose phosphate, MG—monoacylglycerol, FA—fatty acid, GABA—gamma-aminobutanoic acid, RI—retention index.

Article Snippet: Medicago lupulina L. subsp. vulgaris Koch MlS-1 line, characterized by high AM symbiotic efficiency (mycorrhizal growth response (MGR)), was used under conditions of deficiency or abundance of Pi.

Techniques: Control, Variant Assay